Fig 3. Regulation of splenic DC receptor mediated phagocytosis of ovalbumin occurs independently of Ptpn22.

(A-B) Wild type (WT) and Ptpn22^-/- splenocytes were incubated for 45 minutes with ovalbumin-AF647 on ice or at 37°C and stained for lineage^-, CD11c⁺, I-A^b+ DC. (A) Representative flow cytometry staining of CD11c⁺ I-A^b+ splenic DCs (left hand panel, gated on live, singlet, lineage^-) and ovalbumin-AF647 fluorescence in splenic DCs (right hand panel) incubated with ovalbumin-AF647 for 45 minutes on ice (black dashed line) or at 37°C (black solid line). (B) ovalbumin-AF647 Geometric Mean Fluorescent Intensity (GMFI) of WT and Ptpn22^-/- CD11c⁺ I-A^b+ splenic DC incubated at 37°C for 45 minutes. Squares represent individual mice and bars represent the mean ± s.d. (C) Wild type (WT) and Ptpn22^R619W splenocytes were incubated for 45 minutes with ovalbumin-AF647 at 37°C. Circles represent individual mice and bars represent the mean ± s.d. NS = not significant by unpaired T-test.