Skip to main content
. 2017 Oct 17;12(10):e0185976. doi: 10.1371/journal.pone.0185976

Table 1. Summary of positive clones derived from memory B cells.

Antigen ELISA and cell based binding assay
Donor ID Number of positive clones / Total clones (% of positives)
Antigen ELISA Cell based binding assay
MG1 12 / 208 (5.8%) 0 / 208 (0%)
MG2 11 / 414 (2.7%) 0 / 414 (0%)
MG3 11 / 360 (3.1%) 0 / 360 (0%)
MG5 6 / 344 (1.7%) 0 / 344 (0%)
MG7 6 / 269 (2.2%) 0 / 269 (0%)
Healthy control 1 / 250 (0.4%) 0 / 250 (0%)
Total (MG) 46 / 1595 (2.9%) 0 / 1595 (0%)

ID indicates the donor enrolled in this study. Healthy control indicates non-MG PBMC purchased from the Veritas corporation. Number and % of positive clones of antigen (recombinant ECD of the α-subunit) ELISA and DB40 cell based binding assay are shown. Clones that show signal intensity greater than two-fold more than that of an irrelevant protein (BSA) or the parent cell line (TE671) are defined as positives.