Table 1. Summary of positive clones derived from memory B cells.
Antigen ELISA and cell based binding assay | ||
---|---|---|
Donor ID | Number of positive clones / Total clones (% of positives) | |
Antigen ELISA | Cell based binding assay | |
MG1 | 12 / 208 (5.8%) | 0 / 208 (0%) |
MG2 | 11 / 414 (2.7%) | 0 / 414 (0%) |
MG3 | 11 / 360 (3.1%) | 0 / 360 (0%) |
MG5 | 6 / 344 (1.7%) | 0 / 344 (0%) |
MG7 | 6 / 269 (2.2%) | 0 / 269 (0%) |
Healthy control | 1 / 250 (0.4%) | 0 / 250 (0%) |
Total (MG) | 46 / 1595 (2.9%) | 0 / 1595 (0%) |
ID indicates the donor enrolled in this study. Healthy control indicates non-MG PBMC purchased from the Veritas corporation. Number and % of positive clones of antigen (recombinant ECD of the α-subunit) ELISA and DB40 cell based binding assay are shown. Clones that show signal intensity greater than two-fold more than that of an irrelevant protein (BSA) or the parent cell line (TE671) are defined as positives.