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. 2017 Oct 17;12(10):e0186450. doi: 10.1371/journal.pone.0186450

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© 2017 Kondoh et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) To examine whether mlEFL35p interacted with mlEFL35p itself, EBOV and RESTV VP35s, FLAG-tagged mlEFL35p, EBOV and RESTV VP35s (FLAG-ZVP35 and FLAG-RVP35, respectively) expressed in HEK 293T cells were immunoprecipitated (IP) with HA-tagged mlEFL35p and VP35s of EBOV and RESTV (HA-ZVP35 and HA-RVP35, respectively). Precipitated proteins were detected by western blotting with anti-FLAG tag antibodies. (B) EBOV NP (ZNP) and RESTV NP (RNP) were expressed in HEK 293T cells and immunoprecipitated with HA-tagged VP35 of EBOV and RESTV (HA-ZVP35 and HA-RVP35) or HA-tagged mlEFL35p. HA-tagged proteins were detected by western blotting with an anti HA-tag antibody. ZNP and RNP were similarly detected with rabbit antisera specific to NPs.