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Double inmunostaining in Pth4:eGFP transgenic embryos using anti-eGFP antibody (A and B) and anti-HuC/D antibody (A´and B´) shows complete co-localization at 1 and 2 dpf (A´´ and B´´). Ventral views with anterior to the top. Pth4:eGFP: green; HuC/D: red; nuclear stain: blue. Abbreviation: e, eye. Scale bar: 50 μm.
