Fig. 2.

Expression levels of cytochrome P450 family 34/35 (Cyp34/35) mRNA orthologues in response to thiabendazole. Fourth stage larvae obtained by in vitro culture were exposed to 0.05% DMSO (vehicle control) for 3 h or 0.5 μg/ml thiabendazole (TBZ) in 0.05% DMSO for 3 or 6 h. Expression of the Cyps HCOI100383400 (A) HCOI100383700 (B), HCOI01928800a (C), HCOI01928800b (D) was analysed using the housekeeping genes superoxide dismutase (sod), fatty acid retinol binding protein (far) and glyceraldehyde-3P-dehydrogenase (gpd) as reference genes and the HcH isolate as control for normalisation.