Figure 1.

Morphological and Phenotypic Characterization of Freshly Isolated Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids

(A–C) Phase-contrast microscope revealed the morphology of the freshly isolated human pachytene spermatocytes (A), spermatogonia (B), and round spermatids (C) of OA patients. (D–F) DIC microscope showed the morphological characteristics of the freshly isolated human pachytene spermatocytes (D), spermatogonia (E), and round spermatids (F) of OA patients. Scale bars, 20 μm (A–C) and 5 μm (D–F). (G) RT-PCR revealed the transcripts of GPR125, RET, GFRA1, THY1, UCHL1, MAGEA4, and PLZF in the fleshly isolated spermatogonia, the expression of SYCP3 and SYCP1 in pachytene spermatocytes, and mRNA of TNP1, TNP2, PRM1, PRM2, and ACR in round spermatids. RNA without RT (RT-) but with PCR of GAPDH primers was utilized as negative controls, and GAPDH served as loading controls of total RNA.