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. 2017 Sep 25;9:182–194. doi: 10.1016/j.omtn.2017.09.007

Figure 2.

Figure 2

Phenotypic Characterization of Freshly Isolated Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids

(A–D) Immunocytochemistry revealed the expression of THY1 (A), GFRA1 (B), PLZF (C), and UCHL1 (D) in the freshly isolated human spermatogonia. Scale bars, 10 μm. (E) Meiotic chromatin spread by triple immunostaining displayed the co-expression of CREST, SYCP3, and MLH1 in the freshly isolated human pachytene spermatocytes. Scale bar, 5 μm. (F–I) Immunocytochemistry demonstrated the expression of PNA (F), PRM2 (G), and rabbit IgG (I) in the freshly isolated human round spermatids and rabbit IgG (H) in the freshly isolated human spermatogonia. Scale bars, 10 μm.