. 2017 Sep 25;9:182–194. doi: 10.1016/j.omtn.2017.09.007

Table 2.

The Sequences of Gene Primers Used for RT-PCR and Real-Time PCR

Genes	Primer Sequences (5′–3′)		Product Size (bp)	Temperature (°C)
GPR125	forward	CAGAACATTGGCAGGCATTAC	367	62
GPR125	reverse	CATCACGTCACCTAGCTCTTT	367	62
RET	forward	CTCGTTCATCGGGACTTG	126	60
RET	reverse	ACCCTGGCTCCTCTTCAC	126	60
GFRA1	forward	GGACTCCTGCAAGACGAATTA	543	62
GFRA1	reverse	GAAGCACCGAGACCTTCTTT	543	62
THY1	forward	CAGAAGGTGACCAGCCTAAC	233	60
THY1	reverse	TTGCTAGTGAAGGCGGATAAG	233	60
UCHL1	forward	CCCGAGATGCTGAACAAAGT	274	62
UCHL1	reverse	TGGCCACTGCGTGAATAAG	274	62
MAGEA4	forward	CCGAGAAGCACTCAGTAACAA	107	60
MAGEA4	reverse	TGATGACTCTCTCCAGCATTTC	107	60
PLZF	forward	GATCCTCTTCCACCGCAATAG	99	60
PLZF	reverse	TGCAGCGTGGCTGTATATG	99	60
SYCP1	forward	AACTACTGTCTGCAGCTTGG	124	60
SYCP1	reverse	CATCTCTTCCAGCTCACTTGAT	124	60
SYCP3	forward	TGCAGAAAGCTGAGGAACAA	247	58
SYCP3	reverse	TGCTGCTGAGTTTCCATCAT	247	58
TNP1	forward	CGACCAGCCGCAAATTAAAG	110	60
TNP1	reverse	CAGGTTGCCCTTACGGTATT	110	60
TNP2	forward	AGACTCACAGCCTTCCTATCA	359	62
TNP2	reverse	CTGGATCCTCTTGGCCATTT	359	62
PRM1	forward	ATAGCACATCCACCAAACTCC	120	58
PRM1	reverse	AGGCGGCATTGTTCCTTAG	120	58
PRM2	forward	CATGGGCAAGAGCAAGGA	105	60
PRM2	reverse	TCTGCGCCTATAGTGAGACT	105	60
ACR	forward	CATCGACCTGGACTTGTGTAA	369	58
ACR	reverse	CAAGGAAGGTGAGCAGAGATAG	369	58
GADPH	forward	AATCCCATCACCATCTTCC	382	55
GADPH	reverse	CATCACGCCACAGTTTCC	382	55
TGFBR1	forward	ACGGCGTTACAGTGTTTCTG	167	60
TGFBR1	reverse	GCACATACAAACGGCCTATCTC	167	60
AKT3	forward	TGTGGATTTACCTTATCCCCTCA	79	60
AKT3	reverse	GTTTGGCTTTGGTCGTTCTGT	79	60
POU2F1	forward	ATGAACAATCCGTCAGAAACCAG	157	60
POU2F1	reverse	GATGGAGATGTCCAAGGAAAGC	157	60
ETV3	forward	GGTGGAGGGTATCAGTTTCCT	346	60
ETV3	reverse	TGATGAATGGGTAGTTGGGCAT	346	60
NFYC	forward	GGAGGATTTGGTGGTACTAGCA	109	60
NFYC	reverse	GCACTCGGAAGTCTTTCACTG	109	60
RORA	forward	ACTCCTGTCCTCGTCAGAAGA	95	60
RORA	reverse	CATCCCTACGGCAAGGCATTT	95	60
FGFR3	forward	CCCAAATGGGAGCTGTCTCG	109	60
FGFR3	reverse	CCCGGTCCTTGTCAATGCC	109	60
SP1	forward	TGGCAGCAGTACCAATGGC	126	60
SP1	reverse	CCAGGTAGTCCTGTCAGAACTT	126	60
SMAD3	forward	TGGACGCAGGTTCTCCAAAC	90	60
SMAD3	reverse	CCGGCTCGCAGTAGGTAAC	90	60
ACTB	forward	CCTGGCACCCAGCACAAT	144	60
ACTB	reverse	GGGCCGGACTCGTCATAC	144	60