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. 2017 Sep 25;9:182–194. doi: 10.1016/j.omtn.2017.09.007

Table 3.

The Sequences of miRNA-Specific Primers Used for Real-Time PCR

Human MicroRNAs Primer Sequences (5′–3′) Temperature (°C)
hsa-miR-100-5p AACCCGTAGATCCGAACTTGTG 60
hsa-miR-145-3p GGATTCCTGGAAATACTGTTCT 60
hsa-miR-99a-5p AACCCGTAGATCCGATCTTGTG 60
hsa-miR-143-3p TGAGATGAAGCACTGTAGCTC 60
hsa-miR-145-5p GTCCAGTTTTCCCAGGAATCCCT 60
hsa-miR-99b-5p CACCCGTAGAACCGACCTTGCG 60
hsa-miR-373-3p GAAGTGCTTCGATTTTGGGGTGT 60
hsa-miR-105-5p TCAAATGCTCAGACTCCTGTGGT 60
hsa-miR-296-5p AGGGCCCCCCCTCAATCCTGT 60
hsa-miR-423-5p TGAGGGGCAGAGAGCGAGACTTT 60
hsa-miR-181a-5p AACATTCAACGCTGTCGGTGAGT 60
hsa-miR-140-3p TACCACAGGGTAGAACCACGG 60
Let-7e-5p TGAGGTAGGAGGTTGTATAGTT 60
hsa-miR-26a-5p TTCAAGTAATCCAGGATAGGCT 60
hsa-miR-424 CAGCAGCAATTCATGTTTTGAA 60
hsa-miR-186-5p CAAAGAATTCTCCTTTTGGGCT 60
hsa-miR-21-5p CAAAGAATTCTCCTTTTGGGCT 60
hsa-miR-374 TTATAATACAACCTGATAAGTG 60
Let-7b TGAGGTAGTAGGTTGTGTGGTT 60
hsa-miR-31-5p AGGCAAGATGCTGGCATAGCT 60
hsa-miR-10b-5p TACCCTGTAGAACCGAATTTGTG 60
hsa-miR-199b-3p ACAGTAGTCTGCACATTGGTTA 60
hsa-miR-409-5p AGGTTACCCGAGCAACTTTGCAT 60
hsa-miR-127-3p TCGGATCCGTCTGAGCTTGGCT 60
Let-7i-5p TGAGGTAGTAGTTTGTGCTGTT 60
Let-7f-5p TGAGGTAGTAGATTGTATAGTT 60
hsa-miR-221-3p AGCTACATTGTCTGCTGGGTTTC 60
Let-7g-5p TGAGGTAGTAGTTTGTACAGTT 60
hsa-miR-9-3p ATAAAGCTAGATAACCGAAAGT 60
U6 CAAGGATGACACGCAAATTCG 60