Figure 5.
Soluble Innate Factors Are Important for Establishing Protection against Intracranial VSV Infection
(A) BALB/c mice were primed i.p. with virus-depleted serum, bulk splenocytes, or bulk iliac lymph node populations isolated from mice at 6 hr (all tissues) or 24 hr (serum only) post-VSVΔ51-GFP prime or with 1 × 108 PFU VSVΔ51-GFP (positive control) or PBS (negative control). At 24 hr post-prime, mice received an IC dose of 2 × 107 PFU VSVΔ51-fLuc. Replication of the IC dose in the brain was monitored by luciferase signal via IVIS imaging. Dotted line represents background luminescence. Error bars refer to SD. (B) Levels of IFNα, IFNβ, and IFNγ were assessed by ELISA in triplicate samples in the serum or CSF of naive mice at 6 or 12 hr following a single peripheral (i.p.) or IC dose of 1 × 108 PFU VSVΔ51-GFP. Error bars refer to SD. (C) BALB/c mice were primed with IFNα, IFNβ, IFNγ, or 1 × 108 PFU VSVΔ51-GFP (i.v.) (positive control) or PBS (i.v.) (negative control). At 24 hr post-prime, mice received an IC dose of 2 × 107 PFU VSVΔ51-fLuc. IC virus replication was tracked by luminescence using IVIS imaging (left), and corresponding survival data were captured (right) based on 3 mice per group. Dotted line represents background luminescence. Statistical analysis of survival curves was performed using the log-rank (Mantel-Cox) test relative to PBS. *p < 0.05; **p < 0.01; ns, no significance. Error bars refer to SD.