FCU, FDU and tail fascicles were mechanically tested at 3, 6, 15 and 27 months (22-27 months for male FCU) to identify age-related changes in toe stiffness, linear stiffness, transition extension and maximum load. Apart from an increased FCU toe stiffness at 3 months (p=0.027 versus 15 months and p=0.012 versus 27 months) and an increased linear stiffness at 3 months (p=0.0013 versus 9 months, p=0.012 versus 15 months and p=0.011 versus 27 months) in female mice, no age-associated changes in mechanical properties were found. (*) indicates p<0.05.