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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Cell Biochem. 2017 May 30;118(12):4240–4253. doi: 10.1002/jcb.26074

Figure 1. Deletion of the RANKL D5 enhancer reduces matrix calcification in murine aortic smooth muscle cells.

Figure 1

(a) Murine aortic smooth muscle cells (mAoSMCs) isolated from wild-type (WT), Rankl-null (Rank−/−), Rankl transgenic (Rankl−/−;Tg) and D5 enhancer-null (D5−/−) mice were differentiated in either standard medium (SM) or osteogenic medium (OM) for 21 days. Alizarin red S and von Kossa staining were carried out to identify deposited calcium in cell layers following differentiation. (b) Calcium content was quantified by decalcifying the cells with 0.6N HCl. *P < 0.05 versus SM; Student’s t test. UD, Undetected.