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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Cell Biochem. 2017 May 30;118(12):4240–4253. doi: 10.1002/jcb.26074

Figure 2. Deletion of the RANKL D5 enhancer leads to increased bone mass in hypercholesterolemic Apoe-null mice.

Figure 2

(a – f), Total body (a and d), lumber spinal (b and e) and femoral (c and f) bone mineral density (BMD) of WT, D5−/−, Apoe−/− and Apoe−/−;D5−/− mice (n = 7 – 10 per group) fed a high-fat diet (HFD) for either 12 or 18 weeks. (g – l), Expression of mRNA transcripts of Rankl (g–i) and Opg (j–l) in tibiae, spleen and thymus of WT, D5−/−, Apoe−/− and Apoe−/−;D5−/− mice (n = 7 – 10 per group) fed the HFD for 18 weeks, assessed by qRT-PCR and normalized to β-actin. Values represent mean ± s.e.m. *P < 0.05 versus WT, #P < 0.05 versus Apoe−/−, calculated by one-way ANOVA.