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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Cell Biochem. 2017 May 30;118(12):4240–4253. doi: 10.1002/jcb.26074

Figure 3. Deletion of the RANKL D5 enhancer reduces RANKL expression in atherosclerotic lesions.

Figure 3

(a) Figure shows a representative picture of an aorta along with the heart that was cleaned off the extra fat and tissues. The aorta was collected after 18 weeks of a high-fat diet (HFD) feeding. The picture shows the aortic arch (circled in yellow) and thoracic aorta. As can be seen, the aortic arch has extensive plaques appears in white fatty streaks whereas few or no fatty streaks are present in the thoracic aorta region. The aortic arches and thoracic aortas were dissected and the RNA was isolated for gene expression analysis. (b – e) Expressions of mRNA transcripts of Rankl (b), Opg (c), Lgr4 (d) and Rank (e) in the aortic arch and the thoracic aorta of WT, D5−/−, Apoe−/− and Apoe−/−;D5−/− mice (n = 7 – 10 per group) fed the HFD for 18 weeks, assessed by RT-qPCR normalized to β-actin. Values represent mean ± s.e.m. *P < 0.05 versus WT, #P < 0.05 versus Apoe−/−, calculated by one-way ANOVA.