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. 2017 Oct 17;7:13324. doi: 10.1038/s41598-017-13187-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoreactivity of fossil and extant turtle tissues. Immunohistochemical staining results for (a,b,e,f,i,j,m,n,q,r; columns 1 and 2) MHM-K2 and Chelonia mydas (c,d; columns 3 and 4) carapace scute and (g,h,k,l,o,p,s,t; columns 3 and 4) muscle tissue to antibodies raised against (a–d; row 1) Gallus gallus domesticus feathers (anti-Gallus fth), (e–h; row 2) Alligator mississippiensis haemoglobin (anti-Alligator Hb), (i–l; row 3) Struthio camelus haemoglobin (anti-Struthio Hb), (m–p; row 4) G. g. domesticus tropomyosin (anti-Gallus trop), and (q–t; row 5) bacterial peptidoglycan (anti-bac pep). a,c,e,g,i,k,m,o,q,s are overlay images, superimposing fluorescent signal on transmitted light image of sectioned tissue to reveal the localisation of antibody-antigen complexes to tissue. b,d,f,h,j,l,n,p,r,t are imaged using a FITC filter. Antibody-antigen complexes are indicated by green fluorescent signal.