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. 2017 Oct 17;7:13324. doi: 10.1038/s41598-017-13187-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of immunoreactivity between fossil and extant turtle tissues using antibodies raised against chicken feathers conjugated to 12 nm gold beads. (a–d) Low and (e–h) high resolution localisation of gold beads to fibrous matter, but not microbodies/melanosomes, in (a,b,e,f) fossil tissues and (c,d,g,h) modern Chelonia mydas carapace scute material. Insets in b and d demarcate areas depicted in e,f and g,h, respectively. The data support the specificity of the chicken feather antibodies used in this study, and provide independent validation of the immunofluorescent results.