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. 2017 Oct 17;7:13334. doi: 10.1038/s41598-017-13477-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MSC Culture Condition Development. (A) Schematic illustrating the workflow and conditions used to optimize the culture of mMSC. The fresh isolated bone marrow cells were divided into 4 groups: normoxic (20% O₂) or hypoxic (5% O₂) conditions +/−10 ng/mL of bFGF supplement. (B) The representative phase contrast images were acquired (10x) at different time points during the p0 to p1 growth phase: d3, d5, and d7 post-plating. (d14 images found in Fig. 2A). Scale bars = 500 μm.