Table 1.

Top 20 biomarker candidate clusters.

Cluster #	Cluster size (n)	m/z	Chg.	Cluster similarity (p-value)	Subjects (n)	Median change			AUC (C - AD)	AA Sequence	Protein name
						AD - C	MCI-AD - C	MCI-S - C
7367	39	794.115	5	0.073	119	215%**	153%**	35%*	0.96*
69078	18	664.799	2	0.000	94	18%**	15%**	0%	0.92*
32527	11	777.426	3	0.001	68	13%**	8%*	8%	0.92*
78065	41	748.382	2	0.001	120	38%**	20%**	9%*	0.91*	aDSGSSEEkQ	osteopontin
4243	10	696.457	5	0.000	59	103%*	67%**	59%**	0.91*
82223	22	794.885	2	0.000	111	28%**	22%**	11%**	0.90*	tVASHTSDSDVPSG	clusterin
34935	10	810.044	3	0.001	58	16%**	16%*	-1%	0.9*
9084	13	836.597	5	0.000	86	47%**	35%**	27%*	0.87*
36033	34	823.45	3	0.006	120	18%**	13%*	3%*	0.87*	kVEQAVETEPEPELRQQ	ApoE
61266	14	1380.71	3	0.000	70	−20%**	−7%	−10%*	0.86*
1889	53	427.579	3	0.014	120	15%**	8%*	6%	0.86*
33238	10	787.612	3	0.001	69	19%**	34%**	15%*	0.85*
25327	14	684.044	3	0.003	60	9%**	9%*	10%*	0.85*
13290	25	840.651	4	0.038	111	17%**	16%**	7%	0.85*	gGSLPSEEkGHPQEESEESNVSMASLGE	secretogranin-1
10464	9	786.901	4	0.002	59	22%**	4%	8%	0.85
71623	10	685.336	2	0.000	61	13%**	7%*	7%*	0.83*
12243	10	911.104	5	0.000	70	−28%**	−7%	6%	0.82
87816	44	848.511	2	0.002	69	16%**	24%**	6%	0.8*
6405	11	690.794	4	0.000	60	13%*	−2%	−1%	0.79*
53204	8	1088.54	3	0.000	60	4%*	16%*	8%	0.79*

**Indicates statistical significance (p < 0.001), *Indicates statistical significance (p < 0.05). Top 20 spectral clusters sorted according to descending Area Under ROC curve in AD vs C. Columns (left to right) are: cluster id number; number of spectra included in the cluster; cluster average mass-to-charge ratio (m/z); charge; average p value of cluster similarity as calculated by the MS Cluster algortihm; the number of study subjects in which the cluster was quantified; median relative change in abundance between AD and C, between MCI-AD and C, and between MCI-S and C; area under curve from ROC curve analysis of C vs. AD; amino acid sequence of peptides identified by database searching; protein name.