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. 2017 Oct 17;7:13331. doi: 10.1038/s41598-017-13752-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calibration of the wavelength-dependent response in the emission and detection paths. The published two-photon excitation cross section of fluorescein compared to that obtained from the META detector (a) and the calibration curve derived from this data (b). The output spectrum (c) obtained from an LED torch at the wavelengths used within the study when measured by the three fixed filters (320/20, 420/20 and 460/30), spectrometer and META spectral detector and the calibration curve for the META detector derived from this data (d).