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. 2017 Oct 17;7:13351. doi: 10.1038/s41598-017-13867-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Catsper1au is located in vivo in the nucleus of spermatogenic and somatic cells from the seminiferous tubule. (a) Catsper1au RNA was analysed in the mouse testis by FISH using antisense RNA probe derived from Catsper1au. (b) Sense Catsper1au RNA was included as a negative control. Antisense (c) and sense (d) Actb RNA probes were also included as controls. Dashed line, basal border of seminiferous tubule; VI and VIII, stages in the cycle of the seminiferous epithelium; SC: Sertoli cell; sg: spermatogonia cell; ps: pachytene spermatocyte; rs: round spermatid; es: elongated spermatid; sp: sperm cell. Blood vessel: bv; lc: Leydig cell.