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. 2017 Oct 17;7:13365. doi: 10.1038/s41598-017-14000-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of CTL-derived vesicles. Naïve CD8⁺ T cells purified from OT-I mice were stimulated with 2SI (cont) or 3SI (IL-12) for three days in vitro, and extracellular vesicles were purified from each supernatant. (A) Size distribution measured in Malvern Zetasizer Nano ZS90. (B) Concentration of protein and vesicle particles per mL of supernatant. (C) Transmission Electron Microscopy. Purified vesicles were observed under Zeiss EM10 transmission electron microscope. The graphs vary on amplification magnitude, and the bar in each graph indicates 200 nm. Each experiment was replicated at least three times with similar results.