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. 2017 Oct 17;7:13365. doi: 10.1038/s41598-017-14000-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-12 stimulation results in selective enrichment of exosomal proteins in activated CTL-derived exosomes. Purified exosomes from 2SI (control) and 3SI (IL-12) activated CTLs were loaded at 10 μg protein in each lane, and processed together for each protein/marker. Uncropped images of the blots for Fig. 2A were shown in Suppl. Figure 3. (A) Representative blots. Protein expression levels of (B) exosome markers and (C) CTL-associated proteins were compared between cells and exosomes from CTLs. Each value is expressed as the relative expression of proteins in exosomes versus cell lysates after normalization to the relevant GAPDH expression level as an internal control. Data are expressed as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired, two-tailed Student’s t-test.