Figure 1.

P4-Liganded PRA Promotes while P4-Liganded PRB inhibits Gap-Junction Coupling in Uterine Myocytes. (a,b) Representative Cx43 immunofluorescence images in PR inducible hTERT-HM^A/B cell line induced for PRA (a) or PRB (b) expression and stimulated with 100 nM P4 for 24 h. Yellow arrows show major accumulation site of Cx43 (c) Dye transfer assay performed with lucifer yellow (green) and rhodhamine dye (red) in hTERT-HM^A/B cell lines induced for PRA or PRB expression and treated with P4 (100 nM) or vehicle for 24 h. (d) Quantification of scrape loading/dye transfer assay (5 fields, 3 replicates, repeated four times) is shown. Data represents mean ± SD, n = 4. ‘***’ denote statistical significance at p < 0.001, ‘**’ at p < 0.01 and ‘*’ at p < 0.05 with respect to vehicle control in DMSO group.