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. 2017 Oct 17;7:13357. doi: 10.1038/s41598-017-13488-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cx43 forward trafficking from ER to Golgi is blocked in PRB expressing cells. Representative images of immunofluorescence on hTERT-HM^A/B cells induced for PRA (left panel) or PRB (right panel) expression and stimulated with P4 (100 nM). Images show Cx43 in green fluorescence (a,c,e,g), Endoplasmic reticulum (ER) marker(PDI, b,d) and Golgi (G) marker (Golgi-58K9, f,h) in red colour, and co-localization of Cx43 and ER/G in yellow [Cx43 + ER (b,d) and Cx43 + G (f,h)]. PRB expressing cells show accumulation of Cx43 in ER (d, yellow) but not in Golgi (h, red) in the merged images. Scale bar = 20 μm.