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. 2017 Oct 17;7:13357. doi: 10.1038/s41598-017-13488-9

Figure 3.

Figure 3

Cx43-20 K expression is upregulated in the PRA expressing cells and labouring human myometrium. Representative Western blots from (a) human myometrial cells hTERT-HMA/B induced for PRA or PRB expression and stimulated with Vehicle or P4 (100 nM) for 24 h, and (b) human myometrium tissue lysates from term non-labouring (TNIL) and labouring women (TL) pooled from n = 6 samples each. Cx43 full length and Cx43-20 K (20KDa) were detected in the cell line while Cx43-43 K, 32 K, 29 K, 26 K, and Cx43-20 K were detected in the human myometrium tissues using anti-Cx43 (Millipore, Cat # AB1728). ERK2 was used as loading control. (c) Quantitative analysis of Cx43 isoforms in TNIL and TL. Densitometry was performed on western blots for individual TNIL and TL samples (n = 6 per group). Unpaired ttest determined the statistically significant differences in Cx43 isoforms’ expression among TNIL and TL myometrial samples. Data represents mean ± SD, n = 6/group. ‘***’ denote significant increase at p < 0.0001, ‘**’ at p < 0.001 and ‘*’ at p < 0.05 in TL compared to TNIL.