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. 2017 Oct 17;7:13357. doi: 10.1038/s41598-017-13488-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cx43 forward trafficking and Cx43-20 K expression is regulated by mTOR signaling pathway in human myometrial cells. Representative images of hTERT-HM^A/B cells transfected with Cx43-GFP and induced for PRA (a,b,c), or PRB (d,e,f) expression for 24 h and stimulated with P4 (100 nM) for 2 h. Results showed that Cx43 forward trafficking is promoted in PRA cells (a) and restricted in the PRB cells (d) however inhibition of mTOR signaling pathway via specific inhibitor PP242 (5 μM) resulted in the rescue of Cx43 trafficking defect in PRB cells (e) whereas stimulation of mTOR signaling pathway via insulin (10 μg/ml) treatment resulted in the inhibition of Cx43 trafficking in PRA cells (c). Scale bar = 20 μm. Regulation of Cx43-20 K expression in PRB cells by PP242 and insulin is shown in the western blot image (g).