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. 2017 Oct 17;7:13348. doi: 10.1038/s41598-017-13606-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of miR156 precursors in the primary and axillary shoot apices of A. alpina after vernalization. Transcript levels of pre-miR156a, b and c were checked in the plants treated with 12 weeks of vernalizartion after 8-week growth in long days. 8WV, plants vernalized with 12 weeks of cold after 8 weeks of growth in room temperature. VS1 to VS5 indicate S1 to S5 stages of axillary shoot apices from 8WV plants. Primary shoot apices of 3W, 5W, 7W old vegetative plants were compared with axillary branches from 8-week vernalized plants for comparison of miR156 levels.