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. 2017 Oct 17;7:13348. doi: 10.1038/s41598-017-13606-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flowering competence of axillary shoot apices after vernalization in A. alpina depends on developmental stages. (A) Expression levels of AaLFY in the primary and axillary shoot apices undergoing various developmental stages after vernalization (8WV, vernalization-treated plants after 8 weeks growth in long days; VS1 to VS5, axillary branches of S1 to S5 stages from 8WV plants). (B)~(E) Morphologies of shoot apices in different developmental stages. (B,C) VS2 axillary branches show either vegetative (B) or reproductive (C) development. (D) All of VS3 axillary shoot apices and (E) all the primary shoot apices of 8WV plants show inflorescence development.