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. 2017 Oct 17;7:13348. doi: 10.1038/s41598-017-13606-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vernalization response of A. thaliana Sy-0 according to age. (A) Expression levels of pre-miR156a in the primary shoot apices of 7, 10, 20, and 30-day-old Sy-0 before vernalization (black bars) and after 6 weeks vernalization (white bars) treatment. (B) Expression levels of LFY were analyzed before (black bars) and after (white bars) vernalization in Sy-0. Plants in variable ages from 7 days to 30 days old were exposed to 6 weeks vernalization. (C) Flowering time was measured by the number of rosette leaves produced when flowering. Plants in variable ages from 2 days to 30 days old were exposed to 6 weeks vernalziation.