Figure 1.

Screening of Ustilago maydis LAMP primers. The LAMP fluorescence signal (RFU) vs. Cq [threshold time (min)] amplification curve graph was plotted automatically by the Bio-Rad CFX96 Real-Time PCR system. 12 sets of primers that targeted one of three U. maydis effector genes (See1, Pit2 and Pep1) were designed for the primers optimization experiment. 1–4 indicates one of the four sets of primers designed for each gene; NC indicates the reaction using negative control templates (the DNA of another maize pathogens, Sporisorium reiliana, which causes symptoms similar to corn common smut).