Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 17;7:13394. doi: 10.1038/s41598-017-13881-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Specificity test of RealAmp assay for the detection of Ustilago maydis. (a) The RealAmp fluorescence (RFU) vs. Cq [threshold time (min)] amplification curve graph was plotted automatically by the Bio-Rad CFX96 Real-Time PCR system. (b) Visual inspection of the RealAmp amplification products using SYBR Green I. The original orange color of SYBR green turned to green in a reaction mixture showing a positive result. The templates consisted of genomic DNA isolated from the following controls and other pathogens: PC, positive control (Staphylococcus aureus); NC-1, negative control 1 (Salmonella choleraesusi); NC-2, negative control 2 (S. bongori); SG200, Ustilago maydis strain SG-200; 1, Curvularia lunata; 2, Bipolaris maydis; 3, Colletotrichum truncatum; 4, Sporisorium reiliana; 5, Fusarium moniliforme; 6, F. graminearum; 7, Pythium inflatum.