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. 2017 Oct 17;7:13394. doi: 10.1038/s41598-017-13881-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative detection of artificially inoculated soil and maize plant samples using RealAmp and real-time PCR. (a) Detection of Ustilago maydis from serial 10-fold dilutions ranging from 1.6 × 10⁷ to 1.6 × 10² spores per gram of artificially infested soil using RealAmp. The quantitative detection of U. maydis in maize plants challenged with sporidia of U. maydis strain SG200 or compatible mating strains at 1 DPI (day post inoculation) and 6 DPI using RealAmp (b) and real-time PCR (c). Serially diluted template DNA (ranging from 10⁰ to 10⁻³) of 1 DPI were used to test the sensitivity of RealAmp in detecting U. maydis at the early stage of infection in the artificially inoculated maize plants. Approximately 354 ng/μl genomic DNA of U. maydis strain SG200 was employed as a quantitative reference.