Table 1.

[L₁₆(4⁵)] orthogonal design for Ustilago maydis LAMP system

Combination	Factor
	Bst DNA polymerase concentration (U)	inner vs outer primer (μM)	Mg2+ concentration (mM)
1	2	0.8: 0.2	5
2	4	1.2: 0.2	5
3	6	1.6: 0.2	5
4	8	0.4: 0.2	5
5	2	0.4: 0.2	6
6	4	1.6: 0.2	6
7	6	1.2: 0.2	6
8	8	0.8: 0.2	6
9	2	1.6: 0.2	7
10	4	0.8: 0.2	7
11	6	0.4: 0.2	7
12	8	1.2: 0.2	7
13	2	1.2: 0.2	8
14	4	0.4: 0.2	8
15	6	0.8: 0.2	8
16	8	1.6: 0.2	8