Figure 1.

Wolbachia infection status and relationship to mtDNA copy number. (A) Relationship between depth and breadth of sequencing coverage for Wolbachia assemblies in the D. simulans panel. Depth of coverage is shown in log₁₀ unites and is calculated as the number of reads present at each nucleotide in the reference averaged over every site. Breadth of coverage is the proportion of covered nucleotides in the consensus sequence relative to the reference. (B) Relationship between relative mtDNA copy number and Wolbachia copy number. Both were normalized relative to nuclear coverage. Although separate regions were used to normalize mtDNA and Wolbachia, as they are different sizes, average values were very similar within genotypes. The relationship between mtDNA and Wolbachia copy number is positive (p < 2.4 × 10⁻⁷).