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Wolbachia and mtDNA Tajima’s D. 10,000 simulations were performed for Wolbachia and D. simulans each conditioned upon the number of polymorphisms. The actual values in D. simulans mtDNA are outside the 95% confidence interval of the simulations, while Wolbachia is not. There is considerable variation in Tajima’s D across the Wolbachia genome while mtDNA is much smaller and invariant in its values of Tajima’s D.
