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. 2017 Oct 13;11:321. doi: 10.3389/fncel.2017.00321

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Copyright © 2017 Nayler, Powell, Vanichkina, Korn, Wells, Kanjhan, Sun, Taft, Lavin and Wolvetang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptomic characterization of differentiated neurons. (A) Table detailing iPSCs selected for RNA sequencing. (B) Representative iPSC colony immunolabelled with antibody to TRA-1-60. Scale bar is 10 μm. (C) PCA shows tight clustering of experimental replicates. Y-axis shows PC2, X-axis shows PC1. (D) Correlation between samples following differentiation by limma-estimated log-fold change in expression between AT30 d0 vs. d34 and C11 d0 vs. d34. (E,F) Volcano plots show changes in the expression of genes after differentiation (E = AT30 d0 vs. d34, F = C11 d0 vs. d34). Differentially expressed genes (adj. p-value < 0.01 and logFC > 1.5) are shown as blue/red points.