Figure 5.

Immunocytometric measurement of RAW264.7 macrophage cell-line expression of major cell surface antigens F4/80 and CD11b following of the treatment by glycoconjugates GM-1 or GM-2 at 10 or 100 µg/mL concentrations. Gates were set to exclude the cellular debris using forward scatter vs. side scatter dot plot discrimination, doublets and dead cells (A). The settings were optimized using proper isotype control. Fluorescence histograms (B) of 10,000 cells were generated and analyzed (green fluorescence, 525-nm band-pass filter). The data (C) are expressed as a mean of fluorescence intensity. The statistical significance of differences between untreated cells (Control) and stimulated cells using one-way ANOVA and post hoc Bonferroni’s tests is expressed as follows: ***—P < 0.001, **—0.001 < P < 0.01, *—0.01 < P < 0.05.