Table 1.

RAW264.7 macrophage phagocytosis of C. albicans–FITC (%) following 24- and 48-h cell treatment with glycoconjugates GM-1 and GM-2 analyzed by flow cytometry.

Sample	Dose, μg/mL	Cell bound and internalized C. albicans–FITC		Internalized C. albicans–FITC		Membrane attached C. albicans–FITC
		24 h	48 h	24 h	48 h	24 h	48 h
Control		39.3 ± 3.2	56.7 ± 4.2	12.1 ± 1.2	25.8 ± 2.1	27.2 ± 2.5	30.9 ± 1.8
GM-1	10	24.4 ± 2.3	27.4 ± 2.1**	4.8 ± 1.2**	1.6 ± 0.2***	19.6 ± 0.5*	25.8 ± 1.6
	100	21.7 ± 1.9	28.9 ± 1.7**	2.1 ± 0.9**	1.5 ± 0.3***	18.9 ± 0.9*	27.4 ± 0.9
GM-2	10	51.3 ± 3.4**	63 ± 2.9	7.7 ± 0.8	27.8 ± 2.5	43.6 ± 2.2**	35.2 ± 1.7
	100	46.4 ± 2.8*	56.7 ± 3.7	15.7 ± 1.8	24.9 ± 1.8	30.7 ± 1.9	31.8 ± 1.5

Control represents untreated cells (Control). The amount of membrane attached C. albicans–FITC is expressed as a difference between the population of cell membrane attached and cell internalized C. albicans–FITC and Trypan blue unquenched population, i.e., internalized cells. All data are presented as Mean ± SD. Tests were carried out in triplicate. The statistical significance of differences between untreated cells and stimulated cells using one-way ANOVA and post hoc Bonferroni’s tests is expressed as: ***—P < 0.001, **—0.001 < P < 0.01, *—0.01 < P < 0.05.