Skip to main content
. 2017 Jun 5;2:36. [Version 1] doi: 10.12688/wellcomeopenres.11764.1

Figure 2. Consequence of XBP1s expression on XBP1u splicing in cell lines following endoplasmic reticulum stress.

Figure 2.

( A) Schematic of the three possible cDNA products generated by primers that flank the XBP1 spliced intron (indicated in grey). Single stranded cDNA can anneal to either its complementary strand to generate double stranded XBP1u and XBP1s products, or generate a hybrid, XBP1h, formed from one strand XBP1u and one strand XBP1s. Of these three PCR products, XBP1s migrates the furthest on an agarose gel, followed by XBP1u and then by XBP1h, leading to the appearance of three distinct DNA bands. Adapted from 20. ( B– E) XBP1 splice assay of cDNA extracted from either ( B, D) CHO-S or ( C, E) CHO-S XB cells treated with doxycycline (Dox; for 3 days) and/or tunicamycin (Tn; for 3 h) as indicated. PCR reactions contained primers that anneal only to the endogenous CHO XBP1 sequence ( B, C) or primers that anneal to both the endogenous sequence and the exogenous XBP1s transcripts ( D, E). The experiment was performed at least three times, data from a representative experiment is shown.