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XBP1 splice assay of cDNA from CHO-S XB cells induced for 3 days with a range of doxycycline (Dox) concentrations, either not treated with tunicamycin (Tn) ( A, C), or treated with 10µg/mL Tn for 3 h. Primers specific for endogenous XBP1 ( A, B) or endogenous and exogenous XBP1 ( C) were used. The experiment was performed twice, data from a representative experiment is shown.
