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. 2017 Oct 9;2:36. Originally published 2017 Jun 5. [Version 2] doi: 10.12688/wellcomeopenres.11764.2

Figure 5. Differential effect of PERK and Ire1 activation is not due to differences in sensitivity to UPR induction.

Figure 5.

( A) Expression of spliced exogenous XBP1 in CHO XB cells was induced by incubation with 1μg/ml doxycycline for 48 hours (refreshed after 24 hours). The cells were incubated with a tunicamycin (TM) concentration range as indicated for three hours. The samples were analysed for unspliced (XBP1u) and spliced (XBP1s) by RT-PCR, with actin as loading control (markers indicated as base pairs on the right). ( B) In a separate experiment, induced and uninduced CHO XB cells were treated with the same tunicamycin concentration range as in ( A) for three hours and analysed by SDS-PAGE and Western blot with an anti-PERK and anti-actin antibody (molecular weight markers indicated in kDa on the right). ( C and D) Quantification of spliced CHO XBP1 and phospho-PERK of ( A) and ( B), respectively. The results in C) and D) are from a single experiment. The experiments in A) and B) were carried out three times with consistent results.