Table 3.
Observed Ki values of DAB, 26 and 2 and type of inhibition with respect to the different substrates in the reactions catalysed by RMGPa, EcGS and SuSy4, respectively. Values are the mean ± s.e. of three independent experiments
| Target enzyme | Inhibitor | Substrate | Type of Inhibition | Ki |
|---|---|---|---|---|
| RMGPa | DAB | Pi | Competitive | 76 ± 6 nM |
| Glycogen | Competitive | 40 ± 5 nM | ||
| EcGS | 26 ADP | ADPG | Competitive | 346 ± 28 μM |
| Glycogen | Non-competitive | 739 ± 10 μM | ||
| ADPG | Competitive | 87 ± 6 μM | ||
| SuSy4 | 2 UDP | ADPG | Competitive | 353 ± 55 μM |
| Glycogen | Non-competitive | 399 ± 20 μM | ||
| UDPG | Competitive | 145 ± 48 μM |