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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: J Toxicol Environ Health A. 2009;72(9):577–584. doi: 10.1080/15287390802706371

FIG. 1.

FIG. 1

Characterization of recombinant human lens ALDH1A1: (A) 5-µg protein samples were run on a 12% SDS-PAGE gel, and (i) stained with Coomassie blue, (ii) immunoblotted with anti-ALDH1A1 antibodies. Lane 1: protein marker; lanes 2 and 3: protein before and after 12 -h IPTG induction; lane 4: purified ALDH1A1 through Ni-His-tag column. (B) Induction of human lens ALDH1A1 activity by IPTG. Protein extracts were obtained from aliquots of E. coli collected at time points 0, 4, 6, 10, 14, 16, and 24 h after IPTG treatment, and the enzymatic activity of ALDH1A1 was determined using propionaldehyde as the substrate and measured at 340 nm for 5 min by spectrophotometer.