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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: J Toxicol Environ Health A. 2009;72(9):577–584. doi: 10.1080/15287390802706371

FIG. 2.

FIG. 2

Inhibition of human lens ALDH1A1 activity by NO donors: 50 µg ALDH1A1 was incubated with (A) 0.1, 0.2, 0.5, and 1.0 mM SNAP or (B) 50, 100, 150, and 200 µM GSNO or (C) 10, 20, 50, and 100 µM GSSG. After 0, 50, 100, 150, and 200 min, an aliquot of 100 µl was withdrawn to determine enzyme activity with 2 mM NAD+ and 1 mM propionaldehyde at 340 nm for 5 min. The data are represented as percent of unmodified (control) ALDH1A1 activity. Values are mean ± SD.