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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: J Toxicol Environ Health A. 2009;72(9):577–584. doi: 10.1080/15287390802706371

FIG. 3.

FIG. 3

NAD protects GSNO modified human lens ALDH1A1: 100 µM (NAD1), 500 µM (NAD2), and 1 mM (NAD3) of NAD were incubated with 50 µg of ALDH1A1 for 5 min, and then 100 µM GSNO was added. After 0, 5, 10, 20, 50, 100, and 150 min, a 100-µl aliquot was withdrawn to determine enzyme activity with 2 mM NAD+ and 1 mM propionaldehyde at 340 nm for 5 min. The data are represented as percent of unmodified (control) ALDH1A1 activity. Values are mean ± SD.