Fig. 12.

Cln3 ^−/− cells negatively impact WT cells. P0 cortical wild type (WT) and Cln3-deficient (Cln3 ^−/−) neuronal cultures were combined with either WT or Cln3 ^−/− mixed glia cultures to study the impact of these glial cells on neuronal health. A After 7 days of co-culture, WT co-cultures were healthy (a) but Cln3 ^−/− mixed glia appeared to have a detrimental effect when cultured with both WT (b) and Cln3 ^−/− neurons (d) with the latter being more dramatically affected. When WT mixed glia were co-cultured with Cln3 ^−/− neurons, neuronal survival improved. B Significantly less LDH released was observed in WT neuron/WT mixed glia co-cultures compared to Cln3 ^−/− neuron/Cln3 ^−/− mixed glia co-cultures. Scale bar in (A) = 20 μm