Table 2.

Shows malaria diagnosis for 30 whole blood samples with the illumigene malaria assay in comparison with standard microscopy, RDT and PCR

Sample	Illumigene	RDT	Microscopy	PCR		Parasite density (parasites/µL)
				Result	Species
1	Pos	Pos	Pos	Pos	Pf + Po	1,027,798
2	Pos	Pos	Pos	Pos	Pf	292,432
3	Pos	Pos	Pos	Pos	Pv	68,129
4	Pos	Pos	Pos	Pos	Pf	27,731
5	Pos	Pos	Pos	Pos	Pv	7285
6	Pos	Pos	Pos	Pos	Pf	5917
7	Pos	Pos	Pos	Pos	Pf + Po	98
8	Pos	Pos (*)	Pos	Pos	Po	1496
9	Pos	Neg	Pos	Pos	Po	59
10	Pos	Neg	Pos	Pos	Pv	340
11	Pos	Neg	Pos	Pos	Pf	415
12	Pos	Pos	Neg	− (#)	–	–
13	Neg	Neg	Neg	/
14	Neg	Neg	Neg	/
15	Neg	Neg	Neg	/
16	Neg	Neg	Neg	/
17	Neg	Neg	Neg	/
18	Neg	Neg	Neg	/
19	Neg	Neg	Neg	/
20	Neg	Neg	Neg	/
21	Neg	Neg	Neg	/
22	Neg	Neg	Neg	/
23	Neg	Neg	Neg	/
24	Neg	Neg	Neg	/
25	Neg	Neg	Neg	/
26	Neg	Neg	Neg	/
27	Neg	Neg	Neg	/
28	Neg	Neg	Neg	/
29	Neg	Neg	Neg	/
30	Neg	Neg	Neg	/

Parasite density as quantified by microscopy on blood smear

RDT, rapid diagnostic test; PCR, four-primer real-time PCR; Pf, Plasmodium falciparum; Pm, Plasmodium malariae; Po, Plasmodium ovale; Pv, Plasmodium vivax; Pos, positive; Neg, negative. –, missing data; (#), negative result with real-time PCR performed on thin blood smear, possibly due to inferior sample quality; (*), difficult to interpret (weak positive result); /, triple negative results (i.e. negative with RDT, microscopy and illumigene malaria assay) were not sent to the ITM for confirmation with real-time PCR