Fig. 4.

Decreased senescence biomarkers on resveralogue treatment (a) Levels of senescence-associated transcripts CDKN2A and CD248 were assessed in senescent populations of NHDF, MRC5 and HF043 fibroblasts by quantitative reverse transcription PCR. Data are expressed relative to stable endogenous control genes GUSB, IDH3B and PPIA, and normalised to the levels of the individual transcripts in untreated controls (c), 1–6 = resveralogues 1–6. Fold change was calculated for in triplicate for three biological replicates (b) Senescence associated β-galactosidase following treatment with resveralogues 1–6 was determined by manually counting the percentage of SA-β gal positive cells (NHDF, MRC5 and HF043) in each treated or control population. n > 300 for each sample. Statistical significance is indicated by * = p < 0.05, ** = p < 0.005, *** = p < 0.0005 (2 way ANOVA)