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. 2017 Oct 17;16:163. doi: 10.1186/s12943-017-0732-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — SNAI2 transforms normal fibroblasts to a CAF-like state. a and b Representative images and quantification of the cellular cytoskeleton by F-actin staining and SNAI2 immunofluorescence in the MRC5 cells (a). and the ovarian normal fibroblasts (NFs) (b). after transfection with Ade-ctrl or Ade-SNAI2. The nuclei were counterstained with DAPI. c Western blot analysis of PDGFRB, FAP, ACTA2 and SNAI2 in the MRC5 cells and the primary ovarian NFs after a 72 h transfection with Adeno-ctrl or Adeno-SNAI2. GAPDH served as the loading control. d Representative images and quantification of the collagen contraction capacity of the MRC5 cells and the primary ovarian NFs in the Adeno-ctrl and Adeno-SNAI2 transfection group. e Representative images and the quantification of the proliferation index of SKOV3 cells cocultured with MRC5 cells and primary ovarian NFs in the Adeno-ctrl and Adeno-SNAI2 transfection group (**P < 0.01, ***P < 0.001)