Fig. 2.

HOTTIP may regulate SCLC cell proliferation and cell cycle. a A pcDNA3.1-HOTTIP expression vector was transfected successfully into H69 and H446 cells, respectively. b HOTTIP RNAi sequences were transfected into H146 and H446AR cells, respectively. c CCK8 assay was used to detect the effect of HOTTIP on SCLC cell proliferation after HOTTIP overexpression. d CCK8 assay was used to detect the effect of HOTTIP on SCLC cell proliferation after HOTTIP knockdown. e & f Flow-cytometric analysis was used for cell cycle detection after HOTTIP knockdown in H446AR and H146 cells. g & h Flow-cytometric analysis was used for cell cycle detection after HOTTIP overexpression in H446 and H69 cells. *, P < 0.05; **, P < 0.01